3AminoSensor - KIT048

Rapid Test Detecting Aminoglycosides In Milk

 packshot

3AminoSensor is a rapid assay in dipstick format detecting the contamination of milk samples by aminoglycosides molecules. This kit is specific for Neomycin/Paromomycin, (Dihydro)-Streptomycin, and Gentamycin detection.

Principle

3AminoSensor is a competitive test involving generic monoclonal antibodies in one single operation.

The test requires the use of two components. The first component is a microwell containing predetermined amounts of specific antibodies with high affinity for Neomycin/Paromomycin, (Dihydro)-Streptomycin, and Gentamycin molecules, linked to gold particles. The second component is a dipstick made up of a membrane with four capture zones : a "control" line located above the test lines for Neomycin, Streptomycin and Gentamycin molecules.

Drug Residue Test

For a valid test, the upper red control line has to be visible after the second incubation. The other three are the specific “test” lines placed below the control line. The line for Neomycin / Paromomycin is located below the Dihydro- and Streptomycin line while the line related to Gentamycin is located above it. When the reagent from the microwell is re-suspended with a milk sample, all monoclonal antibodies will bind the corresponding analytes if present during the first 3-minute incubation at 40°C. Afterwards, when the dipstick is dipped into the milk, the liquid starts running vertically on the dipstick and passes through capture zones. When the sample is free of antibiotics, a colour development occurs at the specific capture lines, indicating the absence of the targeted analytes in the milk sample. On the contrary, the presence of antibiotics in the sample will not cause any coloured signal to appear at the specific capture lines.

Benefits

  • Rapid
  • Adapted To Specific Regulations
    Sensitive Adapted To Specific Regulations
  • Easily Performed On Site Or In The Lab
    User-Friendly Easily Performed On Site Or In The Lab
  • Reliable And Robust
  • Cost-Effective
  • Simultaneous Detection Of Residues
    Multiple 3x Simultaneous Detection Of Residues

Good to know

Aminoglycosides are one of the major classes of antibiotics used in veterinary medicine to treat animal infections. They are especially effective against gram negative bacteria and are frequently used alone and in association with other antibiotics under intramammarry administration to treat mastisis of dairy lactating cows. The detection of residual antibiotics such as Aminoglycosides in milk is of great concern to farmers, milk industry, regulatory agencies and consumers. The consumption of Aminoglycosides can indeed have several adverse effects (allergic reactions, nephrotoxicity and ototoxicity) and may induce the emergence of multi-resistant bacteria that does not respond to commonly used treatments for human illnesses.

Although some microbial inhibition screening tests have a broad spectrum of antibiotics detection in milk, they are usually unable to detect Aminoglycosides at their European Maximum Residue Limit (MRL) concentrations. In contrast, some existing immunoassays such as ELISA are very sensitive but are time-consuming and specific for only one single Aminoglycoside compound due to the very limited structural similarities between the members of this antibiotic family.

How to use

The test can be used in-situ for daily controls or in laboratories for the analysis of series of milk samples. This test does not require any sample processing, cleaning or extraction.

An instrumental reading is also possible with the ReadSensor device.

Test Procedure

Technical Details

• Catalogue Reference : 3AminoSensor Milk MRL 96 tests - KIT048