2MycoSensor DZ Quanti - KIT071

Quantitative Rapid Test Detecting Deoxynivalenol And Zearalenone In Cereals And Animal Feed

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2MycoSensor Quanti is a rapid assay in dipstick format detecting quantitatively the contamination of cereals (corn, wheat, oat, barley) and animal feed samples by Deoxynivalenol and Zearalenone molecules.

Principle

2-Mycosensor is a competitive test involving specific antibodies with high affinity for DON (Deoxynivalenol) and ZEN (Zearalenone) molecules.

The test requires the use of two components. The first component is a microwell containing predetermined amount of specific antibodies with high affinity for DON and ZEN molecules, linked to gold particles. The second component is a dipstick made up of a membrane with three capture zones : a "control" line located above the test lines for DON and ZEN molecules.

For a valid test, the upper red control line has to be visible after the test. When the reagents from the microwell are re-suspended with the cereal extract, specific antibodies will bind the analytes if present. When the dipstick is dipped into the sample, the liquid starts running vertically on the dipstick and passes through capture zones. When the sample is free of toxins, a colour development occurs at the “test” lines, indicating the absence of DON and ZEN in the cereal sample. On the opposite, the presence of toxins in the sample will not cause the appearance of the coloured signal at the “test” capture lines.

Benefits

  • Simultaneous Detection Of Residues
    Multiple x2 Simultaneous Detection Of Residues
  • Large Detection Range
    Quantitative Large Detection Range
  • Rapid
  • Adapted To Specific Regulations
    Sensitive Adapted To Specific Regulations
  • Easily Performed On Site Or In The Lab
    User-Friendly Easily Performed On Site Or In The Lab
  • Reliable And Robust
  • Cost-Effective

Good to know

Vomitoxin, also known as deoxynivalenol (DON), is a mycotoxin that occurs predominantly in grains such as wheat, barley, oats, rye, and maize, and less often in rice, sorghum, and triticale. The occurrence of deoxynivalenol is associated primarily with Fusarium graminearum (Gibberella zeae) and F. culmorum, both of which are important plant pathogens which cause fusarium head blight in wheat and gibberella or fusarium ear blight in maize. Livestock and farm animals: In animals and livestock, vomitoxin causes a refusal to feed and lack of weight gain when fed above advised levels. (Source)

Zearalenone (ZEN), is a potent estrogenic metabolite produced by some Fusarium and Gibberella species. Several Fusarium species produce toxic substances of considerable concern to livestock and poultry producers. Zearalenone is the primary toxin, causing infertility, abortion or other breeding problems, especially in swine. Zearalenone is heat-stable and is found worldwide in a number of cereal crops, such as maize, barley, oats, wheat, rice, and sorghum. (Source)

To protect human health from exposure to these mycotoxins, the European Commission has recently established regulatory limits for DON and ZEN in cereals and cereal-based foods and feeds

How to use

The test can be used in-situ for daily controls or in laboratories for the analysis of series of samples.

Test Procedure

Technical Details

• Catalogue Reference : 2MycoSensor DZ Quanti Cereals MRL 24 tests - KIT071

• The use of the Readsensor allows an accurate quantification of DON and ZEN contaminations in cereal samples in a range of 200 to 3.000 ppb and from 50 to 750 ppb respectively for DON and ZEN, and until 15.000 and 7.500 ppb with an additional dilution of your cereal extract.

ReadSensor