BEADYPLEX - KIT087

Simultaneous screening of 10 antibiotic families in Porcine, Bovine, Poultry, Egg, Milk and Seafood

 packshot

A powerful analytical tool to prevent the presence of antibiotic residues in the food chain.

 

• 10 antibiotic families

• More than 80 residues

• In 1 single test 

Principle

BEADYPLEX combines simultaneous competitive immunoassays in the same single reaction. The test uses unique reagents comprising mixtures of antibiotic-conjugated beads (assay competitors), broad- range antibiotic binders (receptors and antibodies), and  fluorescent secondary binders. Each bead, individually encoded by its specific size and internal fluorescence, in combination with a primary binder, enables the detection of well-defined groups of antibiotics.

 

BEADYPLEX

 

In a first assay step the beads and primary binders are incubated with the sample extract. In the second assay step the labelled secondary binders detect the remaining primary binders on the beads surface, thus generating the final assay signal. The resulting “beads-binders” complexes are then characterized by the Flow Cytometer, which entails the classification of the beads by discrimination of their sizes and internal fluorescence levels, and the measurement of external fluorescence intensities. The presence of antibiotics from a particular family is identified by a signal decrease for the corresponding encoded bead, with respect to a zero dose control sample.

Benefits

  • Large Detection Range
    Quantitative Large Detection Range
  • Rapid
  • Adapted To Specific Regulations
    Sensitive Adapted To Specific Regulations
  • Easily Performed On Site Or In The Lab
    User-Friendly Easily Performed On Site Or In The Lab
  • Reliable And Robust

Good to know

The misuse of antibiotics in animal farming regularly leads to the presence of residues in edible products. Early detection of these residues is essential to guarantee consumer protection and industrial food processing.

BEADYPLEX is an efficient screening method for the analysis of most relevant veterinary antibiotics in different food commodities, providing family identification in one single analysis per sample.

How to use

Technical Details

Catalogue Reference : BEADYPLEX - 96 tests - KIT087

Content :

  • 2x 1.2 μm filterplate (96 wells)
  • 1x 96 wells microplate
  • 2x Assay buffer bottles
  • 1x Extraction buffer bottle
  • 1x Beads mix vial
  • 1x Primary binders vial
  • 1x Secondary binders vial
  • 2x Positive standard vials