TwinSensor Room Temperature - KIT088

Rapid Test Detecting Betalactams and Tetracyclins - The Two Main Classes Of Antibiotics Contaminants In Milk


TwinSensor is a rapid assay in dipstick format detecting the contamination of milk samples by Betalactams and Tetracyclines molecules at Room Temperature. 


Twinsensor RT (KIT088) is a rapid qualitative test that allows you to simultaneously detect the presence or absence of both ß-lactam and Tetracycline molecules in a milk sample without incubator (i.e. at room temperature).

Twinsensor RT is a competitive test involving specific receptors and antibodies in one single operation. The test requires the use of two components. The first component is a microwell containing predetermined amounts of both receptors and antibodies linked to gold particles. The second is a dipstick made up of a set of membranes with specific capture lines.

When the reagent from the microwell is re-suspended with a milk sample, both receptors and antibodies will bind the corresponding analytes if present during the first 4-minute incubation at room temperature. Afterwards, when the dipstick is dipped into the milk, the liquid starts running vertically on the dipstick and passes through capture zones. If the sample is free of antibiotics, a color development occurs at the ‘test’ lines, indicating the absence of the targeted analytes in the milk sample. On the contrary, the presence of antibiotic(s) in the sample will impair the reagent to color the ‘test’ line. In any case, milk has to migrate at least to the upper part of the dipstick after the second incubation.


  • Simultaneous Detection Of Residues
    Multiple x2 Simultaneous Detection Of Residues
  • Rapid
  • Adapted To Specific Regulations
    Sensitive Adapted To Specific Regulations
  • Easily Performed On Site Or In The Lab
    User-Friendly Easily Performed On Site Or In The Lab
  • Reliable And Robust
  • Cost-Effective
  • No Need To Heat
    Room Temperature No Need To Heat

Good to know

Beta-lactams and Tetracyclines antibiotics are the most widely used antibiotics for the treatment of bacterial infections in dairy cattle. A specific indication for administrating both antibiotic types is infectious mastitis. These drugs are also administered to animals in feed for growth promotion and for collective prophylactic treatment.

The monitoring of Beta-lactams and Tetracyclines in milk is important because of the hypersensitivity of some individuals to these antibiotics and also because of the emergence of antibiotic-resistant strains of bacteria. In addition, the general residual level of antibiotics could alter the efficiency of industrial processing of raw milk toward cheese or other fermented dairy products preparation.

How to use

Technical Details

  • The test can be used in-situ for daily controls or in laboratories for the analysis of series of milk samples.
  • This test does not require any sample processing, cleaning or extraction.
  • An instrumental reading is also possible with the ReadSensor 2 device.
  • The current kit is only compatible with raw cow milk