TwinSensor (Russian Regulation) - KIT034

Rapid Test Detecting Betalactams and Tetracyclins - The Two Main Classes Of Antibiotics Contaminants In Milk

 packshot

TwinSensor is a rapid assay in dipstick format detecting the contamination of milk samples by Betalactams and Tetracyclines molecules

Principle

TwinSensor is a competitive test involving specific receptors with high affinity for Betalactams and Tetracyclines molecules.

The test re­quires the use of two components. The first component is a microwell containing predetermined amounts of both receptors and antibodies linked to gold particles. The second is a dipstick made up of a set of membranes with specific capture lines.

For a valid test, the red control line has to be visible after the second incubation. The ether two are the specific test lines placed on both sides of the control line. The line for B-lactam antibiotics [penicillins and cephalosporins] is located below the "control" while the line related to tetracyclines is located above it. When the reagent from the microwell is re-suspended with a milk sample, both receptors will bind the corresponding analytes if present during the first 3-minutes incubation at 40°C. Afterwards, when the dipstick is dipped into the milk, the liquid starts running vertically on the dipstick and passes through capture zones.

Drug Residue Test

When the sample is free of antibiotics, a colour development occurs at the specific capture lines, indicating the absence of the targeted analytes in the milk sample. On the contrary, the presence of antibiotics in the sample will not cause the coloured signal to appear at the specific capture lines.

Benefits

  • Simultaneous Detection Of Residues
    Multiple x2 Simultaneous Detection Of Residues
  • Rapid
  • Adapted To Specific Regulations
    Sensitive Adapted To Specific Regulations
  • Easily Performed On Site Or In The Lab
    User-Friendly Easily Performed On Site Or In The Lab
  • Reliable And Robust
  • Cost-Effective

Good to know

Beta-lactams and Tetracyclines antibiotics are the most widely used antibiotics for the treatment of bacterial infections in dairy cattle. A specific indication for administrating both antibiotic types is infectious mastitis. These drugs are also administered to animals in feed for growth promotion and for collective prophylactic treatment.

The monitoring of Beta-lactams and Tetracyclines in milk is important because of the hypersensitivity of some individuals to these antibiotics and also because of the emergence of antibiotic-resistant strains of bacteria. In addition, the general residual level of antibiotics could alter the efficiency of industrial processing of raw milk toward cheese or other fermented dairy products preparation.

Maximum residue limits (MRLs) have been specified for food products and milk to control the levels of these antibiotics reaching the consumer. The kit is available in 2 versions :

  • one specific to the European Union maximum residue limits (KIT020)
  • one specific to the Russian Federation (RF) milk limits (KIT034)

How to use

Test Procedure

Technical Details

  • Catalogue Name : TwinSensor Milk BT 10ppb 96 tests - KIT034
  • The test can be used in-situ for daily controls or in laborato- ries for the analysis of series of milk samples.
  • This test does not require any sample processing, cleaning or extraction.
  • An instrumental reading is also possible with the ReadSensor device.